Journal: Glia

Article Title: Single Cell Deletion of the Transcription Factors Trps1 and Sox9 in Astrocytes Reveals Novel Functions in the Adult Cerebral Cortex

doi: 10.1002/glia.24645

Figure Lengend Snippet:

Article Snippet: Goat anti‐mcherry , 1:1000 , Acris/Origene (AB0081‐200).

Techniques:

Journal: Ecotoxicology and environmental safety

Article Title: Additive cardiotoxicity of a bisphenol mixture in zebrafish embryos: The involvement of calcium channel and pump.

doi: 10.1016/j.ecoenv.2023.115225

Figure Lengend Snippet: Fig. 4. Effects of BPs on cardiomyocyte development. Representative confocal microscopy image of TG(myl7:NLS-mCherry) ZFEs at 48 hpf exposed to 0.05% DMSO (A) or Mix 2 (BPA 8.7 µM + BPF 18 µM + BPAF 1.3 µM + BPB 4.2 µM), which induced approximately 40% heart rate reduction (B). BP exposure did not affect the cardiomyocyte count (C), based on the t test (n = 10). BP exposure, which induced approximately 30% heart rate reduction, did not affect mRNA expression of genes related to cardiomyocyte development: hand2 (D) and nkx20.5 (E). Only BPF and BPAF significantly downregulated bmp4 mRNA expression (F), based on the one- way ANOVA test followed by Dunnett’s post hoc analysis (n = 3). SC: DMSO 0.05%, BPA: 25 µM, BPF: 60 µM, BPAF: 4 µM, BPB: 12 µM, Mix 1: BPA 6.6 µM + BPF 13.6 µM + BPAF 1 µM + BPB 3.2 µM. ns, P ≥0.03; *, P ≤0.03; **, P ≤0.002; *** , P ≤0.0002; *** *, P ≤0.0001. The data are presented as means ± standard deviations.

Article Snippet: Embryos were stained with mCherry Mouse Polyclonal Antibody (Cusabio, Houston, TX, USA), Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, and Alexa Fluor Plus 594 (Invitrogen) according to a previously described protocol (Hammond-Weinberger and ZeRuth, 2020).

Techniques: Confocal Microscopy, Expressing

Journal: eLife

Article Title: Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in Drosophila

doi: 10.7554/eLife.89582

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Article Snippet: Antibody , Rabbit polyclonal anti-mCherry , GeneTex , RRID: AB_2721247 ; Cat# GTX128508 , WB(1:1000).

Techniques: Recombinant, In Vitro, Expressing, Activation Assay, Control, Transfection, Construct, Activity Assay, Clone Assay, Knock-In, Mutagenesis, Knock-Out, Kinase Assay, Purification, Reporter Assay, Software, Sequencing

Journal: Scientific Reports

Article Title: Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons

doi: 10.1038/s41598-020-67988-2

Figure Lengend Snippet: TI-VAMP1 and TI-VAMP2 co-travel with DCVs. ( A ) Representative kymographs of neurons co-infected (at DIV 4–6) with TI-VAMP1 and NPY-mCherry (left), or TI-VAMP2 and NPY-mCherry (right), imaged at DIV 11–13. VAMP kymographs in green, NPY kymographs in magenta. The bottom panels are graphic representations of the kymographs to show the quantification of the tracks: VAMP only (green), NPY only (magenta) or co-trafficking/localization (black). ( B ) Percentage moving (diagonal line in kymograph) TI-VAMP1 (n = 16, N = 2) TI-VAMP2 (n = 14, N = 2), and NPY (n = 30, N = 2) puncta. 1-way ANOVA with post-hoc Tukey’s test: VAMP2 vs VAMP1/NPY: p = 0.001 (***). ( C ) Percentage of moving NPY puncta with VAMP1 and VAMP2, and moving VAMP1 and VAMP2 puncta with NPY. 1-way ANOVA: p = 0.48 non-significant (ns). Bars represent mean + SEM. Detailed statistics are shown in Supplementary Table .

Article Snippet: Primary antibodies used were: polyclonal MAP2 (Abcam 1:1,000), monoclonal VAMP2 (Sysy, 1:2000), polyclonal GFP (bioconnect, 1:1,000), polyclonal SCG2 (Biodesign International, 1:500), monoclonal BDNF (DSHB, 1:4), polyclonal synaptophysin 1 (SySy, 1:1,000) polyclonal mCherry (GeneTex, 1:2000), polyclonal HA-tag (Abcam, 1:500).

Techniques: Infection

Journal: Journal of Biomedical Science

Article Title: Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish

doi: 10.1186/s12929-015-0207-2

Figure Lengend Snippet: Constitutively activated MEK1 (MEK1 S219D ) and MEK2 (MEK2 S219D ) both phosphorylated ERK1. a . COS-1 cells were transiently transfected with pcDNA3-HA, pcDNA3-ERK1-HA, pcDNA3-MEK1-mCherry, pcDNA3-MEK1S219D-GFP, pcDNA3-MEK2-GFP, or pcDNA3-MEK2S219D-GFP. Total lysates were analyzed by Western blotting using anti-HA, anti-GFP, anti-pERK monoclonal antibodies; anti-mCherry and anti-Actin polyclonal antibodies. b . Intracellular localization of ERK1, MEK1, and MEK2 in COS-1 cells by fluorescent microscopy. The pcDNA3-ERK1-HA was co-transfected with pcDNA3-GFP (A1-A4), pcDNA3-MEK1-mCherry (B1-B4), pcDNA3-MEK1S219D-GFP (C1-C4), pcDNA3-MEK2-GFP (D1-D4), or pcDNA3-MEK2S219D-GFP (E1-E4). Cy2 or Cy3 dye used an anti-HA monoclonal antibody to detect localization of ERK1 as visualized. DAPI was used to stain nuclear DNA. White arrows indicate the ERK1 protein localized in nuclei and the cytoplasm. IB, immunoblot

Article Snippet: Membranes were blocked with 5 % skim milk in phosphate-buffered saline (PBS) for 1 h at room temperature and then incubated at 4 °C with an anti-HA monoclonal antibody, anti-Actin polyclonal antibody (Santa Cruz, Dallas, TX, USA), and anti-mCherry polyclonal antibody (GeneTex, Hsinchu, Taiwan).

Techniques: Transfection, Western Blot, Bioprocessing, Microscopy, Staining

Journal: Journal of Biomedical Science

Article Title: Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish

doi: 10.1186/s12929-015-0207-2

Figure Lengend Snippet: Transient expressions of MEK1 and MEK2 driven by the krt14 promoter induced papillae formation in skin cells. Lateral view of pTol2-krt14-GFP ( a ), pTol2-krt14-MEK2-GFP ( b ), pTol2-MEK2S223A-GFP ( c ), pTol2-krt14-MEK2S219D-GFP ( d ), pTol2-krt14-MEK1-mCherry ( e ), and pTol2-krt14-MEK1S219D-GFP ( f ) plasmids, which were microinjected into 1-cell stage of zebrafish embryos and visualized at 3 days post-fertilization (dpf). The arrow indicates skin cell papillae and budding in the upper epidermis. The upper panel is a fluorescent image. The middle panel is merged fluorescent and bright-field images. The lower panel is an enlargement of the white box in the middle panel

Article Snippet: Membranes were blocked with 5 % skim milk in phosphate-buffered saline (PBS) for 1 h at room temperature and then incubated at 4 °C with an anti-HA monoclonal antibody, anti-Actin polyclonal antibody (Santa Cruz, Dallas, TX, USA), and anti-mCherry polyclonal antibody (GeneTex, Hsinchu, Taiwan).

Techniques: